Device and method for observing the radiation backscattered by an object
AssigneesCommissariat A L'energie Atomique Et Aux Energies Alternatives, Intelligence Artificielle Applications
InventorsEmmanuelle Schultz, Damien Decq, Michel Roch, Selimen Benahmed
Life of this patent
Estimating the term…
Abstract
A device and method for observing an object, in particular a biological object includes a light source able to illuminate a sample. Under the effect of the illumination, the object emits back-scattered radiation that propagates to a screen, the area of which is larger than 100 cm2. The projection of the back-scattered radiation onto the screen forms an image representative of the back-scattered radiation, called a scattergram. An image sensor allows an image representative of the scattergram formed on the screen to be acquired.
Claims21
A device for observing an object, within a sample, comprising:
- a holder, configured to hold the sample, the holder defining a sample plane, in which the sample lies when the sample is placed on the holder;
- a light source, configured to emit an incident light beam, in order to illuminate the object;
- an image sensor, configured to acquire an image representative of a radiation back scattered by the object when illuminated by the incident light beam;
- a screen, lying facing the holder, so as to be exposed to the radiation back scattered by the object when the object is illuminated by the incident light beam, so as to form, on the screen, a scattergram, the scattergram being the image representative of the back-scattered radiation;
- the screen comprising a first face exposed to the back-scattered radiation, the area of the first face being larger than 100 cm2;
- the image sensor being configured to acquire an image of the scattergram formed on the screen;
- the device further comprising a reflective element, placed between the screen and the object, the reflective element being configured to reflect a portion of the incident light beam along an axis of incidence perpendicular or substantially perpendicular to the sample plane, the reflective element being securely fastened to the first face of the screen;
- the device further comprising a binding medium lying between the reflective element and the screen, the binding medium allowing the reflective element to be fastened to the screen;
- wherein the reflective element and/or the binding medium are configured to absorb at least 50% of the back-scattered radiation propagating between the object and the screen;
- and
- wherein the screen, the reflective element, and the object are aligned along the axis of incidence.
A method for observing an object present in a sample, the sample lying facing a screen, the screen comprising a first face, the method comprising:
- a) illuminating the object using an incident light beam, emitted by a light source, the incident light beam propagating to a reflective element placed between the screen and the object, the reflective element directing all or some of the incident light beam toward the object, the reflective element being joined to the first face of the screen;
- b) exposing the first face of the screen to a light radiation back scattered by the sample under the illumination, so as to form, on the first face, a scattergram, representative of the back-scattered radiation, the area of the first face being larger than 100 cm2;
- c) acquiring an image of the scattergram formed on the screen with an image sensor;
- wherein:
- the incident light beam propagates from the reflective element to the object along an axis of incidence;
- the back-scattered radiation propagating toward the screen, along the axis of incidence, is absorbed before reaching the screen, so as to form a shadow in the scattergram formed on the screen;
- and
- the screen, the reflective element, and the object are aligned with respect to the axis of incidence.
Dependencies are inferred from the claim text.