Double-flowering dwarf Calibrachoa

A method of producing a Calibrachoa plant comprising a double-flowering characteristic and a dwarf growth characteristic, comprising the steps of:

• (i) crossing a first female Calibrachoa plant with a first male Calibrachoa plant to produce F1 plants, wherein said first female Calibrachoa plant comprises a mitochondrial allele associated with at least one SNP mutation selected from the group consisting of (i) a G to C nucleotide substitution at position number 320 of SEQ ID NO:
• 1 and (ii) an A to C nucleotide substitution at position number 247 in SEQ ID NO:
• 1 and exhibiting a double-flowering characteristic, and wherein said first male Calibrachoa plant has at least one copy of a nuclear, recessive allele associated with a SNP mutation consisting of a G to C nucleotide substitution at position 43 of SEQ ID NO:
• 2, wherein, when said nuclear allele is in the homozygous form plants exhibit a dwarf growth characteristic;
• (ii) screening said F1 plants for the presence of said nuclear SNP mutation;
• (iii) selecting an F1 female plant exhibiting said double-flowering characteristic and further comprising at least one copy of said nuclear SNP mutation;
• (iv) crossing said F1 female plant with said first male or a second male Calibrachoa plant having at least one copy of said nuclear SNP mutation to produce F2 plants;
• (v) screening said F2 plants for the presence of said nuclear SNP mutation;
• and
• (vi) selecting an F2 plant exhibiting said double-flowering characteristic and being homozygous for said nuclear SNP mutation.