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US 11,155,845 B2Granted
Method for producing theanine
AssigneeKyowa Hakko Bio Co., Ltd.
InventorsKazuhiko Tabata, Shoto Ohno
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Abstract
According to the present invention, theanine can efficiently be produced without exogenously adding ethylamine and without accumulation or leftover of ethylamine as a byproduct, by using a microorganism having enhanced activity to produce ethylamine with acetaldehyde and alanine as substrates and having enhanced activity of γ-glutamylmethylamide synthetase or glutaminase.
Claims10
A microorganism that has been transformed with:
- [1] a polynucleotide encoding a polypeptide (i) having ethylamine-producing activity to produce ethylamine with acetaldehyde and alanine as substrates and (ii) comprising (a) the amino acid sequence of SEQ ID NO:
- 2, 4, 6, or 8, (b) the amino acid sequence of SEQ ID NO:
- 2, 4, 6, or 8 in which no more than 1-20 amino acids have been deleted, substituted, inserted, or added, or (c) an amino acid sequence that has 95% or more sequence identity to the amino acid sequence of SEQ ID NO:
- 2, 4, 6, or 8, and
- [2] a polynucleotide encoding a γ-glutamylmethylamide synthetase,
- wherein the microorganism produces acetaldehyde, alanine, glutamic acid, and ATP from a carbon source, and
- wherein the microorganism produces theanine.
4 more elements
Dependencies are inferred from the claim text.