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US 11,155,844 B2Granted
Process for the production of ortho-aminobenzoic acid and/or aniline from fermentable substrate using recombinant yeast
AssigneeCovestro Deutschland AG
InventorsGernot Jäger, Amgad Salah Moussa, Simon Klaffl, Thomas Hamedinger, Wolf Kloeckner, Swantje Behnken
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Abstract
The present invention relates to the production of o-aminobenzoic acid from fermentable substrates using yeast cells.
Claims10
A method for producing o-aminobenzoic acid comprising the steps of
- a) fermenting at least one fermentable substrate in a fermentation vessel using a recombinant yeast cell comprising a knock out of the trp4 gene encoding anthranilate phosphoribosyltransferase and having no anthranilate phosphoribosyl transferase activity capable of converting the at least one fermentable substrate into o-aminobenzoic acid, wherein the fermentation in method step a) is performed at a pH between 2.0 and 5.0;
- and
- wherein said recombinant yeast cell produces more o-aminobenzoic acid than the wild type;
- and
- b) separating the produced o-aminobenzoic acid from the fermentation broth.
2 more elements
Dependencies are inferred from the claim text.