Recombinant bacteria for producing 3-hydroxy propionic acid, preparation method therefor, and applications thereof
AssigneeInstitute of Microbiology, Chinese Academy of Sciences
InventorsWeifeng Liu, Bo Liu, Qianqian Cui, Guang Zhao, Yong Tao
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Abstract
Provided is a method for construction of recombinant bacteria for producing 3-hydroxypropionic acid. The method includes: knocking out fadR, fabF and fabH genes of recipient bacteria, introducing acc genes or gene clusters, alKL and Mcr genes, and enhancing the expression of fadL, fadD, sthA genes and atoSC gene clusters in the recipient bacteria. Also provided is a method for producing 3-hydroxypropionic acid by using the recombinant bacteria.
Claims19
A recombinant bacteria that contains a fadR gene, a fabF gene, and a fabH gene, the bacteria comprising a genetic modification that increases content of a protein encoded by a malonyl-CoA reductase gene mcr gene and/or that enhances activity of the protein encoded by the mcr gene.
Dependencies are inferred from the claim text.