Back to results
US 11,155,836 B2Granted
Compositions and methods for monitoring in real-time construction and bioengineering of mammalian synthetic chromosomes
AssigneeCarryGenes Bioengineering, LLC
InventorsEdward Perkins, Amy Greene
Life of this patent
Estimating the term…
Abstract
The present invention encompasses compositions and methods to allow one to monitor formation of synthetic chromosomes in real-time via standardized fluorescent technology, eliminating the need for cumbersome, expensive, and possibly mutagenic analysis.
Claims21
A method for screening production of synthetic chromosomes in living eukaryotic host cells, using a real-time tracking system having at least two tags, the method comprising:
- transfecting real-time tracking system components into a synthetic chromosome production eukaryotic host cell line, wherein the tracking system components comprise:
- (i) an endogenous chromosome tag that labels one or more endogenous chromosomes in the chromosome production host cell line;
- (ii) a differently-labeled synthetic chromosome-specific tag that identifies, apart from the endogenous chromosomes, a synthetic chromosome as it is being produced;
- and
- monitoring the production of the synthetic chromosome in the living synthetic chromosome production eukaryotic host cells in real-time by detecting the endogenous chromosome tag and the synthetic chromosome tag.
2 more elements
Dependencies are inferred from the claim text.