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US 11,155,835 B2Granted
Prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells
AssigneeThe Catholic University of America
InventorsVenigalla B. Rao, Jingen Zhu
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Abstract
Described is hybrid viral vector comprising: a first virus such as bacteriophage T4; one or more second virus such as adeno-associated virus (AAV) attached to the first virus through cross-bridges, such as avidin-biotin cross-bridges; one or more DNA molecules packaged in the first virus; one or more nucleic acid molecules packaged in the second virus; and one or more proteins displayed on the surface of the first virus. Also described are methods of making and using such a hybrid viral vector.
Claims27
A product comprising:
- a first viral vector;
- a second viral vector;
- and
- a cross-bridge connecting the first viral vector to the second viral vector
- wherein the product is a hybrid viral vector and has the ability to infect a host subject or a host cell in the form of hybrid viral vector.
2 more elements
A product comprising:
- a T4 head capsid;
- a biotinylated AAV;
- and
- a biotin-avidin cross-bridge
- wherein the biotin-avidin cross-bridge is Soc-biotin-avidin (SBA) or Hoc-BAP-biotin-avidin (HBBA);
- wherein avidin of the cross-bridge attaches to the biotin on the biotinylated AAV and Hoc or Soc attaches to T4.
3 more elements
A product comprising:
- a first viral vector;
- one or more DNA molecules packaged in the first viral vector;
- a second viral vector;
- one or more nucleic acid molecules packaged in the second viral vector and
- across-bridge connecting the second viral vector to the first viral vector,
- wherein the product is a hybrid viral vector and has the ability to infect a host subject or a host cell in the form of hybrid viral vector.
3 more elements
A method comprising:
- constructing SBA or HBBA cross-bridge;
- attaching the Soc or Hoc end of the cross-bridge to T4;
- biotinylating AAV;
- and attaching the avidin end of the cross-bridge to AAV,
- wherein the SBA cross-bridge is constructed by sequentially connecting Soc, biotin and avidin,
- wherein the HBBA cross-bridge is constructed by sequentially connecting Hoc, biotin acceptor peptide (BAP), biotin and avidin.
3 more elements
A method comprising:
- designing a cross-bridge;
- attaching one end of the cross-bridge to a first viral vector;
- and
- attaching the other end of the cross-bridge to a second viral vector,
- wherein the resulting product is a hybrid viral vector and has the ability to infect a host subject or a host cell in the form of hybrid viral vector.
2 more elements
Dependencies are inferred from the claim text.