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US 11,155,833 B2Granted
Method for producing transgenic cell
AssigneeKaneka Corporation
InventorsTomoyuki Nakaishi, Tatsuya Moutai, Hiroshi Kita, Mitsuaki Kitano
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Abstract
A method for producing a transgenic cell, may include contacting, in vitro, (i) a virus comprising: a chimeric protein of an antibody-binding protein and a vesicular stomatitis virus G (VSV-G) protein; and a foreign gene, with (ii) a target cell and an antibody specific to the target cell, and/or a target cell comprising a membrane antibody, to infect the target cell with the virus.
Claims17
A method for producing a transgenic cell, comprising:
- (a) contacting, in vitro, an antigen of a target cell with an antibody specific to the target cell;
- and then contacting, in vitro:
- (i) a virus comprising:
- a chimeric protein of an antibody-binding protein and a vesicular stomatitis virus G (VSV-G) protein;
- and a foreign gene, with
- (ii) the antibody specific to the target cell that is in contact with the antigen of the target cell, and/or,
- (b) contacting, in vitro:
- (i) the virus comprising:
- the chimeric protein of the antibody-binding protein and the vesicular stomatitis virus G (VSV-G) protein;
- and the foreign gene, with
- (ii) a membrane antibody of a B cell, to infect the target cell with the virus.
8 more elements
Dependencies are inferred from the claim text.