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US 11,155,796 B2Granted
Compositions and methods for epigenome editing
AssigneeDuke University
InventorsCharles A. Gersbach, Isaac Hilton
Life of this patent
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Abstract
Disclosed herein are CRISPR/Cas9-based gene activation systems that include a fusion protein of a Cas9 protein and a protein having histone acetyltransferase activity, and methods of using said systems.
Claims13
An isolated polynucleotide encoding a fusion protein comprising two heterologous polypeptide domains,
- wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein,
- wherein the second polypeptide domain comprises a p300 histone acetyltransferase effector domain, and
- wherein the fusion protein comprises the polypeptide sequence of SEQ ID NO:
- 140, SEQ ID NO:
- 141, or SEQ ID NO:
- 149.
3 more elements
An isolated polynucleotide encoding a fusion protein comprising two heterologous polypeptide domains,
- wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein,
- wherein the second polypeptide domain comprises a p300 histone acetyltransferase effector domain, and
- wherein the first polypeptide domain comprises the polypeptide sequence of SEQ ID NO:
- 10 and the second polypeptide domain comprises the polypeptide sequence of SEQ ID NO:
- 3, or the first polypeptide domain comprises the polypeptide sequence of SEQ ID NO:
- 1 and the second polypeptide domain comprises the polypeptide sequence of SEQ ID NO:
- 2.
4 more elements
Dependencies are inferred from the claim text.