Glucan kinases and methods for processing starch using the same
AssigneeUniversity of Kentucky Research Foundation
InventorsMatthew Gentry, Craig VanderKooi
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Abstract
Provided herein are a glucan kinase polypeptide, an isolated polynucleotide, and a method for processing starch. The glucan kinase polypeptide comprises an isolated polypeptide including a sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO:22, fragments thereof, variants thereof, and combinations thereof. The isolated polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 21, a fragment thereof, a variant thereof, and a combination thereof. The method for processing starch comprises providing a glucan dikinase; exposing a starch to the glucan dikinase; and collecting the starch that has been exposed to the glucan dikinase.
Claims13
A fragment of a non-native glucan kinase polypeptide comprising:
- an isolated polypeptide including a sequence selected from the group consisting of a fragment of SEQ ID NO:
- 2, a fragment of SEQ ID NO:22, and combinations thereof;
- wherein SEQ ID NO:
- 2 and SEQ ID NO:
- 22 are wild-type sequences;
- wherein the fragment is selected from the group consisting of SEQ ID NO:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, and 46;
- and
- wherein the fragment has glucan dikinase activity.
Dependencies are inferred from the claim text.