Bile salt hydrolase probe and method of making and using the same
AssigneeBattelle Memorial Institute
InventorsAaron T. Wright, Kristoffer R. Brandvold, Susan Ramos-Hunter
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Abstract
Probe embodiments for targeting, identifying, and isolating enzymes exhibiting BSH activity as well as devices and kits that use the probes are described herein. Methods of making and using the probes, devices, and kits are also described. In some embodiments, probes, devices, and kits for targeting, identifying, and isolating enzymes in a biological sample are disclosed. In some embodiments, compositions and methods of treatment using the probes, devices, and kits disclosed herein are described.
Claims20
A probe having a structure satisfying Formula I
- wherein each of R1, R2, R3, and R4 independently is hydrogen or —OR, wherein each R independently is hydrogen, a counterion that balances a negative charge on the oxygen atom of the —OR group, or heteroaliphatic;
- R5 is hydrogen or aliphatic;
- the linker is aliphatic or heteroaliphatic;
- DM is (i)
- or (ii) biotin;
- pDM is an alkyne or an azide;
- PM comprises a benzophenone group or a diazirine group;
- AM is an alkyne, an azide, an activated ester, a carboxylic acid, a halide, or an alkyl halide;
- n is an integer ranging from 0 to 10;
- and m is 0 or 1.
A probe having one of the following sctructures
- wherein q is an integer ranging from 0 to 10.
A kit, comprising:
- a substrate;
- and
- a probe having a structure satisfying Formula I
- wherein each of R1, R2, R3, and R4 independently is hydrogen or —OR, wherein each R independently is hydrogen, a counterion that balances a negative charge on the oxygen atom of the —OR group, or heteroaliphatic;
- R5 is hydrogen or aliphatic;
- the linker is aliphatic or heteroaliphatic;
- DM is a fluorophore, a chromogen, or biotin;
- pDM is an alkyne or an azide;
- AM is an alkyne, an azide, an activated ester, a carboxylic acid, a halide, or an alkyl halide;
- n is an integer ranging from 0 to 10;
- and m is 1;
- and wherein the probe is covalently bound to a surface of the substrate via the AM group.
A probe having a structure satisfying Formula I
- wherein each of R1, R2, R3, and R4 independently is hydrogen or —OR, wherein each R independently is hydrogen, a counterion that balances a negative charge on the oxygen atom of the —OR group, or heteroaliphatic;
- DM is
- or biotin;
- and n is an integer ranging from 0 to 10.
A probe having one of the following structures:
Dependencies are inferred from the claim text.