Screening assays and methods
AssigneePresident and Fellows of Harvard College
InventorsJ. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
Life of this patent
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Abstract
Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.
Claims16
An apparatus comprising a moldable slab in contact with a substrate, said moldable slab defining a plurality of wells, wherein each well has a side or diameter of less than 100 microns and contains not more than a single or a few cell(s), wherein at least one of said wells contains the respective single or few cell(s), said respective single or few cell(s) having produced one or more cell-derived product(s) in a volume no greater than about 10 nanoliters in each said well containing said respective single or few cell(s), said one or more cell-derived product(s) being bound to the substrate at a location in fluid communication with said respective well containing said respective single or few cell(s).
Dependencies are inferred from the claim text.