Production of haploid lolium
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorBryan K. Kindiger
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Abstract
A method for producing haploid Lolium plants may start with providing a Lolium multiflorum inducer line, the L. multiflorum inducer line having the ability to induce mitotic genome instability and haploid sectoring when hybridized as a maternal parent with a Lolium sp. paternal parent, such as previously disclosed lines IL1 and IL2. The inducer line may then be crossed with a Lolium sp. to generate F1 plants, and the F1 plants may be self-fertilized so as to recover seed from the selfed plant. The recovered seed may then be planted to generate one or more F2 plants, and at least one of the F2 plants may be a haploid Lolium plant.
Claims5
A method for producing haploid Lolium plants, the method comprising:
- providing a Lolium mutiflorurn inducer line, the L. multiflorum inducer line having the ability to induce mitotic genome instability and haploid sectoring when hybridized as a maternal parent with a Lolium sp. paternal parent;
- crossing said L. multiflorum inducer line with a Lolium sp. to generate F1 plants;
- self-fertilizing at least one of the generated F1 plants;
- recovering and planting seed from the at least one self-fertilized F1 plant to generate one or more F2 plants;
- and
- growing said one or more F2 plants,
- wherein at least one of the one or more F2 plants is a haploid Lolium plant.
Dependencies are inferred from the claim text.