System and method for phase contrast microscopy imaging

A system for phase contrast microscopy imaging, comprising:

• a microscope with an optical axis, wherein the microscope comprises an objective;
• a light source array comprises a fixture and two light source groups, wherein the fixture is disposed on the object side of the objective and has a through hole, and the two light source groups is disposed on the side of the fixture away from the objective and is arranged around the through hole, wherein the two light source groups respectively emit a first beam and a second beam symmetrically arranged about the optical axis to illuminate a specimen, and wherein the first beam and the second beam enter and get scattered in the specimen and are obliquely transmitted through the specimen, and collected by the microscope after passing through the through hole;
• an image acquisition module that is disposed on the image side of the microscope, for acquiring a first image corresponding to the first light beam and a second image corresponding to the second light beam;
• a calculation module for restoring primary color components of the first image and the primary color components of the second image and subtracting the primary color components obtained from the first image and the primary color components obtained from the second image to obtain a phase contrast image;
• wherein the light source groups comprise a plurality of light sources, and the light sources in one light source group and the light sources in the other are arranged symmetrically about the optical axis;
• wherein two symmetrical light sources in the light source groups are monochromatic light sources, the other light sources in the light source groups are visible light sources or monochromatic light sources, and the other light sources in different light source groups that are symmetrical about the optical axis are the same.