Method of DNA synthesis
AssigneeTouchlight IP Limited
InventorsPaul Rothwell, Neil Porter, Lydia Viney, Thomas Adie
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Abstract
The present invention relates to an in vitro cell-free process for production of deoxyribonucleotides (DNAs) comprising at least one hairpin, corresponding DNA products and uses thereof, and oligonucleotides and kits useful in the process of the invention.
Claims13
A single-stranded circular DNA comprising at least one hairpin, wherein the hairpin comprises a portion of a protelomerase target sequence, and wherein the portion is at least 14 bases in length.
A single-stranded circular DNA comprising at least one hairpin, wherein the hairpin comprises at least 14 consecutive bases of a protelomerase target sequence, the protelomerase target sequence being selected from bacteriophage N15 TelN of SEQ ID NO:
- 15, Agrobacterium tumefaciens TelA of SEQ ID NO:
- 20, or Vibrio parahaemolyticus plasmid Vp58.5 of SEQ ID NO:
- 47.
A single-stranded circular DNA comprising at least one hairpin, wherein the hairpin comprises a portion of a protelomerase target sequence, and wherein the portion is at least 14 bases in length and is a perfect inverted repeat.
Dependencies are inferred from the claim text.