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AssigneeSouth China Botanical Garden, Chinese Academy of Sciences
InventorsXuewu Duan, Lu Xiao, Guoxiang Jiang, Yueming Jiang, Huiling Yan, Zhiwei Li, Jing Zeng, Xiaochun Ding
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The present invention provides an ascorbate peroxidase mutant MaAPX1M36K and an application thereof, belonging to the technical field of biotechnology. An amino acid sequence of the mutant MaAPX1M36K of the present invention is shown in SEQ ID NO. 3. In the present invention, the mutant MaAPX1M36K reconstruction protein is obtained by the method of prokaryotic expression. It is found that the mutant MaAPX1M36K improves catalytic efficiency by nearly 5 times, providing a technical reference for further study and application of APX1.
A mutant of Musa acuminata ascorbate peroxidase 1 with a methionine at position 36 is mutated to lysine (MaAPX1M36K), wherein the amino acid sequence of the mutant MaAPX1M36K is shown in SEQ ID NO:
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