Compositions and methods for generating reversion free attenuated and/or replication incompetent vaccine vectors
AssigneesBioVentures, LLC, The Research Foundation for The State University of New York
InventorsJames Forrest, Gang Li, Laurie Krug, Steven Skiena
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Abstract
Provided herein are methods for generating reversion free attenuated and/or replication incompetent vaccine vectors and their use in vaccine compositions and vaccination. In particular, the use of a codon shuffled helper gene is used to produce compositions comprising replication incompetent virus.
Claims20
A method of preventing or reducing viral reversion of a virus during culture, the method comprising:
- a) stably expressing a codon shuffled helper gene in a host cell, wherein the helper gene is codon modified to reduce expression of the helper gene in the host cell relative to the expression of an unmodified helper gene;
- and
- b) culturing the host cell under conditions conducive to the propagation of a replication incompetent virus, wherein the helper gene is deleted from the virus thereby rendering the virus replication incompetent.
A method of producing a viral stock composition, the method comprising:
- a) stably expressing a codon shuffled helper gene in a host cell, wherein the helper gene is codon modified to reduce expression of the helper gene in the host cell relative to the expression of an unmodified helper gene;
- b) culturing the host cell under conditions conducive to the propagation of a replication incompetent virus, wherein the helper gene is deleted from the virus thereby rendering the virus replication incompetent;
- and
- c) collecting the replicated virus.
A vaccine composition, wherein the vaccine composition comprises virus produced by a method comprising:
- a) stably expressing a codon shuffled helper gene in a host cell, wherein the helper gene is codon modified to reduce expression of the helper gene in the host cell relative to the expression of an unmodified helper gene;
- b) culturing the host cell under conditions conducive to the propagation of a replication incompetent virus, wherein the viral genome comprises the deleted helper gene thereby rendering the virus replication incompetent;
- c) collecting the replicated virus;
- and
- d) forming the vaccine composition.
Dependencies are inferred from the claim text.