Microglia derived from pluripotent stem cells and methods of making and using the same
AssigneeNew York Stem Cell Foundation, Inc.
InventorsPanagiotis Douvaras, Scott Noggle, Stephen Chang, Valentina Fossati
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Abstract
The present invention provides methods and compositions for the generation of microglial progenitor cells and microglial cells from pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells. The present invention also provides cells produced using such methods, and both methods of treatment and methods of drug screening that use such cells. Also provided are various tissue culture media, tissue culture media supplements, and kits useful for the generation of human microglial progenitor cells and human microglial cells.
Claims17
A method for generating microglial cells comprising:
- a) culturing pluripotent stem cells in a cell culture medium under conditions that induce myeloid differentiation, leading to the generation of CD14+ and/or CX3CR1+ microglial progenitor cells, wherein culturing comprises contacting the pluripotent stem cells of the cell culture medium with a first composition comprising BMP4 in mTeSR1 media without lithium chloride, GABA, pipecolic acid, bFGF or TGFβ1 and culturing the pluripotent stem cells in the presence of the first composition for approximately 4 days;
- and
- b) culturing the CD14+ and/or CX3CR1+ microglial progenitor cells in a first microglial differentiation medium comprising IL-34, or comprising IL-34 and GM-CSF, thereby generating microglial cells,
- wherein the CD14+ and/or CX3CR1+ microglial progenitor cells produced in (a) are isolated prior to culturing the CD14+ and/or CX3CR1+ microglial progenitor cells in (b).
Dependencies are inferred from the claim text.