Three-dimensional bioreactor for T-cell activation and expansion for immunotherapy

A method for T-cell expansion comprising:

• supplying a 3D bioreactor of biocompatible polymeric material comprising a plurality of voids having a surface area for cell expansion, said plurality of voids having a diameter D, a plurality of pore openings between said voids having a diameter d, such that D>d and wherein:
• (a) 90% or more of said voids have a selected void volume (V) that does not vary by more than +/−10.0%;
• and (b) 90% or more of said pore openings between said voids have a value of d that does not vary by more than +/−10.0%;
• providing a polydopamine coating on said bioreactor surface area for cell expansion;
• providing a tetrameric protein attached to said polydopamine coating;
• providing one or more biotinylated antibodies immobilized on said tetrameric protein;
• flowing T-cells through said bioreactor having T-cell receptors where said T-cell receptors bind to said one or more biotinylated antibodies and are activated;
• exposing the activated T-cells to a perfusion media containing a signaling molecule to promote T-cell expansion.