Method for producing low-sugar vegetable and fruit enzyme product
AssigneeGreenyn Biotechnology Co., Ltd
InventorsPang-Kuei Hsu, Yu-Cheng Lin, Chia-Feng Wu
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Abstract
This invention disclosed a method for preparing low-sugar vegetable and fruit enzyme product comprising obtaining a fruit or/and vegetable as a material; fermenting the material for at least three times sequentially, and producing an enzyme product, wherein the first fermentation is yeast fermentation, the second fermentation is acetic acid bacteria fermentation and the third fermentation is lactic acid bacteria fermentation; and the sugar content of the enzyme product is less than 5 wt %, especially, in a predetermined fermentation condition, the sugar content of the enzyme product is less than 2.5 wt %.
Claims8
A method for producing low-sugar vegetable and fruit enzyme product, consisting of:
- a. preparing a starting medium including at least one vegetable and fruit, adding a sugar to obtain the starting medium with 15 to 25° Brix;
- b. preparing an active yeast of Saccharomyces cerevisiae strain at a first inoculated amount of 0.01˜1% w/v to mix with the starting medium for anaerobic fermentation until a residual sugar content is less than 2% and an alcohol content is from 4.4 to 5.5%, so that a first fermented product is generated;
- c. preparing an acetic acid bacteria of Acetobacter aceti strain at a second inoculated amount of 1˜10% w/v to mix with the first fermented product for aerobic fermentation in 35˜40° C. until an acetic acid concentration is from 0.05 to 0.46%, so that a second fermented product is generated;
- d. preparing a Lactobacillus sporogenes at a third inoculated amount of 5˜20% w/v to mix with the second fermented product for aerobic fermentation until a lactic acid content is greater than 600 ppm and a content of total sugar including both the added sugar in step a and the residual sugar is less than 2.5%, so that a third fermented product is generated;
- and
- e. filtering the third fermented product to obtain the low-sugar vegetable and fruit enzyme product.
Dependencies are inferred from the claim text.