Methods and kits for screening transplant recipients and candidates
AssigneeNational Institute of Transplantation Foundation
InventorsJames C Cicciarelli, Noriyuki Kasahara
Life of this patent
Estimating the term…
Abstract
Methods and kits are provided for determining of immunoglobulin isotypes and subclasses in a subject. In general the subject is a human who is a transplant candidate recipient or recipient, has allergies, or has an autoimmune disease. The method involves analyzing a sample of a body fluid of a transplant candidate or recipient, allergy patient or autoimmune disease sufferer and correlating the relative amounts of each immunoglobulin isotype and subtype, such that the distribution of isotypes and subtypes is an indication of success of the transplant in the candidate and recipient or the prognosis of the autoimmune disease.
Claims20
A kit for generating a time series of quantified amounts of IgG1 and IgG4 for a subject comprising:
- beads for generating the time series;
- human leucocyte antigens for generating the time series that comprise at least one human leucocyte antigen bindable by IgG1 and at least one human leucocyte antigen bindable by IgG4;
- at least one monoclonal anti-subject antibody capable of specifically binding to IgG1 and at least one monoclonal anti-subject antibody capable of specifically binding to IgG4 for generating the time series;
- detectable labels for generating the time series, wherein a human subject sample that comprises IgG1 and IgG4 forms bead bound complexes detectably labeled to quantify an amount of the IgG1 and an amount of the IgG4 in the human subject sample;
- and
- instructions for use to relate the time series of quantified amounts of IgG1 to C4d reactivity and quantified amounts of IgG4 to at least one blocking function and to relate the time series to a transplantation prognosis of the subject.
Dependencies are inferred from the claim text.