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US 11,143,650 B1Granted
Method for measurement of total protein content and detection of protein via immunoassay in a microfluidic device
AssigneeProteinSimple
InventorsIrina Georgievna Kazakova, Crystal Tran, Jessica Dermody, Annegret Boge, Tom Yang
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Abstract
Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.
Claims17
A method, comprising:
- electrophoretically separating a sample containing a plurality of proteins in a microfluidic device;
- immobilizing the plurality of proteins in the microfluidic device after electrophoretic separation;
- introducing, into the microfluidic device, a molecule with a reactive moiety configured to non-specifically bind to proteins;
- introducing, into the microfluidic device, a primary antibody configured to bind to at least a subset of proteins from the plurality of proteins;
- introducing, into the microfluidic device, a secondary antibody configured to bind to the primary antibody;
- detecting the subset of proteins based on an optical characteristic associated with the secondary antibody;
- introducing, into the microfluidic device, a stripping reagent configured to remove the primary antibody from the subset of proteins;
- introducing, into the microfluidic device, an optically detectable agent configured to bind to the molecule;
- detecting an optical signal associated with the optically detectable agent;
- and
- normalize the optical characteristic associated with the secondary antibody based on the optical signal associated with the optically detectable agent.
8 more elements
Dependencies are inferred from the claim text.