Free-solution response function interferometry
AssigneeVanderbilt University
InventorsDarryl J. Bornhop, Amanda Kussrow, Michael Kammer
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Abstract
Disclosed are methods for the free solution measurement of molecular interactions by refractive index sensing other than backscattering interferometry. The disclosed methods can have very low detection limits and/or very low sample volume requirements. Also disclosed are various biosensor applications of the disclosed techniques. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
Claims19
An analytical method comprising detecting a molecular interaction product between a first analyte and a second analyte, wherein the detection is performed by refractive index sensing other than backscattering interferometry, or by circular dichroism, wherein one of the analytes is immobilized, and wherein one of the analytes is present in an amount of less than about 1.0×10−6 M.
An analytical method comprising the steps of:
- (a) providing a refractive index sensor for reception of a fluid sample to be analyzed;
- (b) introducing a first sample comprising a first analyte to be analyzed and a second sample comprising a second analyte to be analyzed onto the sensor, wherein the first analyte is allowed to interact with the second analyte;
- (c) interrogating the fluid sample with light;
- (d) detecting the light after interaction with the fluid sample, wherein the detected light is not backscattered;
- and
- (e) detecting a molecular interaction between the first and second analyte,
- wherein one of the analytes is immobilized, and
- wherein one of the analytes is present in an amount of less than about 1.0×10−6 M.
A system comprising a refractive index sensor for detecting a molecular interaction product between a first non-immobilized analyte and a second analyte, a pressure change compensator, and a first non-immobilized analyte in an amount of less than about 1.0×10−6M, wherein detecting is performed by refractive index sensing other than backscattering interferometry.
Dependencies are inferred from the claim text.