US 11,143,633 B2 · Systems, methods and devices for decreasing solubility problems in chromatography

Abstract

A significant reduction in extra-column band broadening can be achieved by decoupling the injection system from the main solvent flow line. In addition, by decoupling the injection system from the main solvent flow line, additional components (e.g., filters, valves, etc.) can be introduced into the chromatography system without increasing the negative band broadening effects. Systems and methods herein provide not only for such decoupling but also for filtering precipitates from the system. As a result, a larger volume of sample can be injected into the present systems without compromising separation yield. In addition, an increase the column loading per batch, an increase the overall yield of separations, and greater system efficiency (i.e., less time lost for cleaning and maintenance) can be realized.

Claims12

Dependent claims

A chromatography system comprising:

a first co-solvent source in fluid communication with a first mixer;
a second co-solvent source and a sample source in fluid communication with a second mixer;
a mobile phase source configured to provide mobile phase to the first and second mixers;
a strainer to minimize precipitation of sample in mobile phase;
a sample loop positioned downstream and in fluid communication with the strainer;
a chromatography column;
and
a valve, the valve having a plurality of discrete positions forming different fluidic connections including (i) a first position in which the first mixer is in fluid communication with the chromatography column and the second mixer is in fluid communication with the chromatography column.

5 more elements

Dependencies are inferred from the claim text.

Systems, methods and devices for decreasing solubility problems in chromatography

Life of this patent

Systems, methods and devices for decreasing solubility problems in chromatography

Life of this patent

Abstract

Claims12