Systems and methods for monitoring lifelong tumor evolution field of invention

Abstract

Disclosed are for monitoring tumor load in a patient by selecting a predetermined number of biomarker genes from DNA extracted from a tumor tissue sample from the patient to form a panel of biomarker genes (“customized genes”); isolating circulating cell-free DNA from a bodily fluid (body fluid) sample of the patient; enriching DNA sequences containing the biomarker genes in the cell-free DNA fragments; sequencing the enriched DNA; counting the number of mutated DNA and normal DNA sequencing reads in enriched DNA; and obtaining a tumor load of the patient. Optionally, the detection of mutations in genes related to therapeutic treatments (“medicine genes”) are carried out simultaneously with the testing of customized genes.

Dependencies are inferred from the claim text.