Back to results
US 11,142,791 B2Granted
Combined multiple-displacement amplification and PCR in an emulsion microdroplet
AssigneeThe Regents of the University of California
InventorsAdam R. Abate, David Sukovich
Life of this patent
Estimating the term…
Abstract
The methods and systems described herein provide an improved emulsion droplet based nucleic acid amplification method, which allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., polymerase chain reaction (PCR). The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA.
Claims20
A nucleic acid amplification method comprising:
- (a) encapsulating a target nucleic acid and amplification reagents in an emulsion microdroplet, wherein the amplification reagents comprise non-specific amplification reagents and PCR amplification reagents;
- (b) non-specifically amplifying the target nucleic acid, thereby producing a non-specific amplification product in the emulsion microdroplet;
- (c) amplifying the non-specific amplification product of (b) by PCR amplification, thereby producing a PCR amplification product in the emulsion microdroplet;
- and
- (d) detecting the PCR amplification product of (c).
2 more elements
Dependencies are inferred from the claim text.