Microfluidic device

A microfluidic device for PCR amplification comprising:

• a plurality of wells, each well comprising one opening to function as both an inlet and an outlet for the well,
• wherein each opening is in fluid communication with a common fluidic channel, and wherein
• each opening is connected to the common fluidic channel via an isolation channel, and
• wherein the plurality of wells are arranged on the device in a radially symmetrical pattern,
• wherein a first end of the common fluidic channel is dimensioned to be fluidly connected with one or more of the following sources via one or more connections:
• a source comprising a possible target molecule, a sealant source, a washing reagent source and other sources for reagents used for polymerase chain reactions or enzyme-linked immunosorbent assays,
• wherein a second end of the common fluidic channel is dimensioned to be fluidly connected with a vacuum pump or a syringe;
• and
• wherein one or more liquid flow stoppers are connected between the second end of the fluidic channel and the vacuum pump or the syringe, wherein the stopper comprises holes sized to prevent liquid from passing through, but allow gas to pass through.