Mass spectrometric resistance determination by measuring metabolism
OwnerMarkus Kostrzewa, Katrin Sparbierinventors
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Abstract
The invention relates to a mass spectrometric method to determine microbial resistances to antibiotics. The decrease or modification of specific nutrient components by microbes, and thus the metabolism of the microbes, is determined mass spectrometrically in culture media containing antibiotics. Hence it is not the microbes which are introduced into the mass spectrometric analysis, but the culture medium. The special nutrient components which are subject to the mass spectrometric observation are indicators for the metabolism exhibited by the microbes in the culture in the presence of antibiotics, and are thus indicators for their susceptibility or resistance.
Claims10
A method for the mass spectrometric determination of microbial resistance to an antibiotic, the method comprising:
- pre-culturing microbes in a culture medium containing a specific concentration of the antibiotic,
- adding, after said pre-culturing step, at least one further nutrient to the culture medium;
- and
- determining mass spectrometrically whether the at least one further nutrient of the culture medium decreases during subsequent culturing or a chemically modified variant of the at least one further nutrient increases during subsequent culturing, whereby a decrease in the at least one further nutrient or an increase in the chemically modified variant of the at least one further nutrient indicates resistance to the antibiotic at the given concentration.
A method for the mass spectrometric determination of resistance of microbes to an antibiotic, comprising:
- growing the microbes in a culture medium which contains a specific concentration of the antibiotic and at least one nutrient peptide containing a core peptide of D-amino acids;
- and measuring by mass spectrometry to determine whether the core peptide of D-amino acids is increased.
Dependencies are inferred from the claim text.