Enhanced production of rhamnolipids using at least two carbon sources

A method for producing one or more rhamnolipids, said method comprising:

• (a) culturing a rhamnolipid producing microorganism in culture medium comprising at least two carbon sources, wherein said carbon source is a sweetener and an oil containing medium chain or long chain triglycerides, at least one nitrogen source, at least one phosphorous source, at least one magnesium source, at least one potassium source, at least one sulfur source, at least one chloride source, at least one sodium source and optionally an emulsifier, for at least about 1 day, wherein said one or more rhamnolipids is obtained at a rate of at least about 1.5 g RL/L/hr;
• (b) clarifying said culture medium to obtain a clarified broth comprising said one or more rhamnolipids;
• and
• (c) measuring rhamnolipid titer of said one or more rhamnolipids from (b) to be at least about 40 g/L.