Decarboxylase and method for producing unsaturated hydrocarbon compound using same
AssigneeRiken
InventorsTomokazu Shirai, Yutaro Mori
Life of this patent
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Abstract
With the aim of providing a method allowing production of an unsaturated hydrocarbon compound such as butadiene with high productivity and an enzyme used in the method, the present inventors introduced mutations involving amino acid replacement into various positions of a ferulic acid decarboxylase, and prepared multiple modified forms of the enzyme. Then, the present inventors evaluated those modified forms in terms of the catalytic activity for the production of butadiene, and found as a result that the catalytic activity was improved in the case where, for example, the amino acid at position 395 was glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine.
Claims4
A method for producing an unsaturated hydrocarbon compound represented by the following formula (2) or a geometric isomer thereof, comprising:
- decarboxylating an unsaturated hydrocarbon dicarboxylic acid compound represented by the following formula (1) or a geometric isomer thereof in the presence of a ferulic acid decarboxylase in which an amino acid at position 395 of an amino add sequence set forth in SEQ ID NO:
- 2 or corresponding to the position is glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine
- [In formulas (1) and (2), R1 and R2 each independently represent a hydrogen atom, a linear or branched alkyl group having 1 to 5 carbon atoms, a linear or branched alkoxy group having 1 to 5 carbon atoms, or hydroxyl group. “A” represents an optionally substituted linear hydrocarbon group having 0 to 5 carbon atoms, and may have a double bond formed between adjacent carbon atoms in the case of 2 to 5 carbon atoms.].
A method for producing an unsaturated hydrocarbon compound represented by the following formula (5) or a geometric isomer thereof, comprising:
- decarboxylating an unsaturated hydrocarbon dicarboxylic acid compound represented by the following formula (3) or a geometric isomer thereof in the presence of a ferulic acid decarboxylase in which an amino acid at position 395 of an amino acid sequence set forth in SEQ ID NO:
- 2 or corresponding to the position is glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine
- [In formulas (3) to (5), R1, R2, R3, and R4 each independently represent a hydrogen atom, a linear or branched alkyl group having 1 to 5 carbon atoms, a linear or branched alkoxy group having 1 to 5 carbon atoms, or hydroxyl group. “A” represents an optionally substituted linear hydrocarbon group having 0 to 5 carbon atoms, and may have a double bond formed between adjacent carbon atoms in the case of 2 to 5 carbon atoms.].
A method for producing an unsaturated hydrocarbon compound, comprising:
- culturing a host cell introduced with a DNA or a vector containing the DNA, the DNA encoding a ferulic acid decarboxylase in which an amino acid at position 395 of an amino acid sequence set forth in SEQ ID NO:
- 2 or corresponding to the position is glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine;
- and collecting an unsaturated hydrocarbon compound represented by the following formula (2) or (5) or a geometric isomer thereof produced in the host cell and/or a cultured product thereof,
- [In formulas (2) and (5), R1, R2, R3, and R4 each independently represent a hydrogen atom, a linear or branched alkyl group having 1 to 5 carbon atoms, a linear or branched alkoxy group having 1 to 5 carbon atoms, or hydroxyl group. “A” represents an optionally substituted linear hydrocarbon group having 0 to 5 carbon atoms, and may have a double bond formed between adjacent carbon atoms in the case of 2 to 5 carbon atoms.].
Dependencies are inferred from the claim text.