Method for preparing mutant strain having high producibility of phytoene and mutant strain prepared thereby

A method for preparing a Deinococcus species mutant strain having a deletion of DR0861 gene using cre-lox system, which comprises the following steps:

• 1) deleting DR0861 gene by introducing a DNA construct in which the upstream and downstream fragments of DR0861 gene were fused to both ends of the lox nucleic acid fragment containing a first selection marker in a Deinococcus species strain, wherein the lox nucleic acid fragment is a polynucleotide composed of the nucleotide sequence represented by SEQ ID NO:
• 17;
• 2) deleting the first selection marker by introducing a vector comprising a groE promoter, a gene encoding cre recombinase, a second selection marker and a temperature sensitive repUts in the strain having deletion of DR0861 gene of step 1), wherein the vector is a polynucleotide composed of the nucleotide sequence represented by SEQ ID NO:
• 21;
• and
• 3) eliminating the vector comprising the second selection marker by culturing the prepared mutant strain obtained in step 2).