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US 11,142,770 B2Granted
Isolated oleaginous yeast
AssigneeMassachusetts Institute of Technology
InventorsGregory Stephanopoulos, Kangjian Qiao, Peng Xu
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Abstract
Some aspects provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. Such engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.
Claims15
An isolated Yarrowia lipolytica cell comprising:
- (a) a nucleic acid construct comprising an expression cassette comprising a nucleic acid sequence encoding a cytosolic NADP+-dependent malic enzyme having the amino acid sequence of SEQ ID NO:
- 2 under the control of a heterologous promoter;
- and
- (b) a nucleic acid construct comprising an expression cassette comprising a nucleic acid sequence encoding a NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase having the amino acid sequence of SEQ ID NO:
- 4 or SEQ ID NO:
- 6 under the control of a heterologous promoter,
- wherein the heterologous promoter is an intron-enhanced TEF promoter or an intron-enhanced Yarrowia lipolytica glyceraldehyde-3-phosphate dehydrogenase promoter.
4 more elements
An isolated Yarrowia lipolytica cell comprising:
- a nucleic acid construct comprising an expression cassette comprising a nucleic acid sequence encoding an aldehyde dehydrogenase (AldH) having the amino acid sequence of SEQ ID NO:
- 30, SEQ ID NO:
- 32, SEQ ID NO:
- 34, SEQ ID NO:
- 36, or SEQ ID NO:
- 38 under the control of a heterologous promoter,
- wherein the heterologous promoter is an intron-enhanced TEF promoter or an intron-enhanced Yarrowia lipolytica glyceraldehyde-3-phosphate dehydrogenase promoter.
4 more elements
Dependencies are inferred from the claim text.