U1 snRNP regulates gene expression and modulates oncogenicity
AssigneeThe Trustees of the University of Pennsylvania
InventorsGideon Dreyfuss, Lili Wan, Jung Min Oh, Anna Maria Pinto, Ihab Younis, Michael George Berg, Daisuke Kaida
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Abstract
The invention provides a method of regulating U1 activity associated with its splicing role as well as its role in protecting pre-mRNAs from premature termination by cleavage and polyadenylation, thereby modulating expression of a gene or genes. In one embodiment, the invention includes compositions and methods for regulating gene expression and treating diseases associated with dysregulated gene expression.
Claims6
A method of increasing the length of more than one shortened transcript in a cell, the method comprising contacting a cell comprising more than one shortened transcript with an activator of endogenous U1 snRNP (U1), wherein one or more of endogenous U1 activity or U1 level associated with protecting a nascent transcript is increased in the cell, permitting an increase in the length of more than one transcript in a cell, wherein an increase in one or more of U1 activity and U1 level results in mRNAs with longer 3′ untranslated regions, wherein the elongated region is the region of the 3′ UTR prior to the polyA tail.
Dependencies are inferred from the claim text.