Acetyl-CoA carboxylase variants
AssigneeGenomatica, Inc.
InventorsDerek L. Greenfield, Donald E. Trimbur, Andreas W. Schirmer, Cindy Chang, Behnaz Behrouzian, Jessica Winger
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Abstract
The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.
Claims15
A variant operon comprising a genetically modified accBC promoter that controls expression of a biotin carboxyl carrier protein (BCCP), wherein the promoter is selected from any one of SEQ ID NOS:
- 93, 94, 95, and 96, wherein the operon results in an increase in BCCP expression in a recombinant microbial cell as compared to a corresponding wild type microbial cell, and wherein the operon confers to the recombinant microbial cell improved production of a malonyl-CoA-derived compound when compared to the corresponding wild type microbial cell.
A recombinant microorganism having increased expression of a nucleic acid sequence comprising accB or accC or a combination thereof, wherein the increased expression is due to one or more genetically modified accBC promoters that drive expression of the nucleic acid sequence, wherein the genetically modified promoter is selected from any one of SEQ ID NOS:
- 93, 94, 95, and 96, and wherein the increased expression results in an increased production of a malonyl-CoA-derived compound when the microorganism is cultured with a carbon source.
Dependencies are inferred from the claim text.