Beta-galactosidase mutant, and preparation method and application thereof
AssigneeJiangnan University
InventorsJing Wu, Dan Wu, Xin Gao
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Abstract
The present invention discloses a β-galactosidase mutant, and a preparation method and application thereof, belonging to the fields of gene engineering and enzyme engineering. Amino acids of specific sites in the β-galactosidase are mutated, the β-galactosidase is transferred into a recombinant bacterium, and enzymatic transformation is performed under optimized conditions, so that the yield of galactooligosaccharide produced by the mutant reaches 59.8%, which is increased by about 70% as compared with that of wild enzyme, thereby implementing the increase of the galactooligosaccharide yield. The present invention has very high industrialized application value.
Claims3
A gene coding a mutant β-galactosidase enzyme comprising a variant of the amino acid sequence of SEQ ID NO:2 with one or more mutations selected from N140C, F264W, F304Q, and W806F, wherein the enzyme catalyzes conversion of lactose to galactooligosaccharide (GOS).
Dependencies are inferred from the claim text.