Saccharide oxidase, and production method for same and use of same
AssigneeAmano Enzyme Inc.
InventorsMiho Nagaya, Akiko Sugita, Naoki Matsumoto, Masamichi Okada
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Abstract
A protein having a novel saccharide oxidase activity capable of being subjected to various uses is provided. The present invention provides a protein having the following physicochemical characteristics: (1) effect: oxidizing a saccharide to produce a saccharic acid; (2) substrate specificity: acting on glucose, maltotriose, maltose, galactose, maltotetraose, lactose, and cellobiose; and, (3) [Km value of glucose]/[Km value of maltose]≤1.
Claims3
A method of producing a saccharic acid in a composition, the method comprising mixing a composition containing saccharide with a manufactured protein having saccharide oxidase activity in the presence of oxygen wherein the protein having saccharide oxidase activity consists of at least one amino acid sequence selected from
- (a) the amino acid sequence SEQ ID NO:
- 10 or
- (b) an amino acid sequence that has at least 90% sequence identity to the amino acid sequence SEQ ID NO:
- 10,
- wherein said protein having saccharide oxidase activity has been collected from a culture producing said protein.
Dependencies are inferred from the claim text.