BCMA-targeted chimeric antigen receptor as well as preparation method therefor and application thereof

A method of treating multiple myeloma associated with expression of a B-cell maturation antigen (BCMA), the method comprising administering to a subject in need thereof a therapeutically effective amount of an engineered T cell comprising a nucleic acid sequence encoding a BCMA-directed chimeric antigen receptor (CAR), wherein the engineered T cell expresses on the surface an anti-BCMA antigen binding region comprising:

• a light chain variable region (VL) having an amino acid sequence set forth in SEQ ID NO:
• 1, and a heavy chain variable region (VH) having an amino acid sequence set forth in SEQ ID NO:
• 2;
• wherein VL is located at the N-terminus of VH,
• wherein the anti-BCMA antigen-binding region is a single-chain variable fragment (scFv) that specifically binds BCMA,
• wherein the CAR further comprises:
• (a) a signal peptide having an amino acid sequence set forth in SEQ ID NO:
• 9,
• (b) a hinge region having an amino acid sequence set forth in SEQ ID NO:
• 12,
• (c) a transmembrane domain having an amino acid sequence set forth in SEQ ID NO:
• 13,
• (d) a co-stimulatory region having an amino acid sequence set forth in SEQ ID NO:
• 14, and
• (e) a cytoplasmic signaling domain having an amino acid sequence set forth in SEQ ID NO:
• 15.