Serum albumin-binding immunoglobulin variable domains
AssigneeAblynx N.V.
InventorMarie-Ange Buyse
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Abstract
The present invention relates to amino acid sequences binding to serum albumin. In particular, the present invention relates to improved immunoglobulin single variable domains (also referred to herein as “ISV's” or “ISVD's”), and more in particular improved heavy-chain immunoglobulin single variable domains, binding to serum albumin, as well as to proteins, polypeptides and other constructs, compounds, molecules or chemical entities that comprise such improved serum albumin binders.
Claims11
An immunoglobulin single variable domain that binds to serum albumin, comprising:
- a CDR1 according to Abm that is the following sequence:
- GFTFDTSSML (SEQ ID NO:13);
- and
- a CDR2 according to Abm that is the following sequence:
- VIHQSGTPTY (SEQ ID NO:14);
- and
- a CDR3 according to Abm that is the following sequence:
- FPSSRMKFDY (SEQ ID NO:
- 15);
- and having:
- an amino acid residue L or V at position 11;
- and
- an amino acid residue T, V or L at position 89;
- and
- an amino acid residue T, K or Q at position 110;
- and
- an amino acid residue S, K or Q at position 112;
- such that (i) position 89 is T;
- or (ii) position 89 is L and position 11 is V;
- or (iii) position 89 is L and position 110 is K or Q;
- or (iv) position 89 is L and position 112 is K or Q;
- or (v) position 89 is L and position 11 is V and position 110 is K or Q;
- or (vi) position 89 is L and position 11 is V and position 112 is K or Q;
- or (vii) position 11 is V and position 110 is K or Q;
- or (viii) position 11 is V and position 112 is K or Q, and wherein the positions are according to Kabat numbering.
Immunoglobulin single variable domain that binds to serum albumin, wherein the immunoglobulin single variable domain comprises an amino acid sequence that is chosen from the amino acid sequences of SEQ ID NOs:
- 72 to 99.
Dependencies are inferred from the claim text.