Glucagon derivative, conjugate thereof, composition comprising same, and therapeutic use thereof

A method for treating congenital hyperinsulinism comprising administering to a subject in need thereof, an isolated conjugate comprising a peptide moiety and a biocompatible material moiety which is linked to the peptide moiety, wherein the peptide moiety comprises the amino acid sequence of the following General Formula 2:

• (SEQ ID NO:
• 46)
• Y-Aib-QGTF-X7-SD-X10-S-X12-Y-L-X15-X16-X17-R-A-
• X20-X21-F-V-X24-W-L-M-N-T-X30 General Formula 2
• wherein
• X7 is threonine (T), valine (V), or cysteine (C);
• X10 is tyrosine (Y) or cysteine (C);
• X12 is lysine (K) or cysteine (C);
• X15 is aspartic acid (D) or cysteine (C);
• X16 is glutamic acid (E) or serine (S);
• X17 is lysine (K) or arginine (R);
• X20 is glutamine (Q) or lysine (K);
• X21 is aspartic acid (D) or glutamic acid (E);
• X24 is valine (V) or glutamine (Q);
• and
• X30 is cysteine (C) or is absent,
• with the proviso that the amino acid sequence of General Formula 2 identical to SEQ ID NO:
• 12 is excluded.