Protein against fungal pathogens
AssigneeNational Institute of Plant Genome Research
InventorsDurga Madhab Swain, Sunil Kumar Yadav, Gopaljee Jha
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Abstract
The present invention relates to a novel protein comprising novel genes that is extracted from Burkholderia gladioli strain NGJ1. A nucleotide sequence encoding the novel protein is represented by sequence SEQ ID No. 1 and amino acid sequence of the novel protein is represented by the sequence SEQ ID No. 2 is further provided. A nucleotide sequence and the amino acid sequence are obtained from genetically engineered Bg_9562 gene. The novel protein as well as encoding gene is adapted for broad spectrum anti-fungal and mycophagous activities.
Claims12
A novel protein comprising an amino acid sequence of SEQ ID No. 2 wherein said amino acid is encoded by the nucleotide sequence of SEQ ID No. 1.
A method to prepare novel protein for anti-fungal activity represented by sequence SEQ ID No. 2, wherein the method comprising the steps of:
- (i) identifying the protein that possess antifungal activity and mycophagous activity from wild Burkholderia gladioli strain NGJ1,
- (ii) analyzing amino acid sequence of the encoded protein by finding critical residues associated with anti-fungal activity as well as mass production, and artificially designing gene sequence to incorporate desired change in the identified protein of step (i) and synthesizing the designed gene through gene synthesis,
- (iii) over expressing evolved protein of step (ii) in an expression vector to get recombinant protein,
- (iv) purifying the over expressed protein of step (iii) by chromatography,
- (v) assessing the antifungal activity of purified protein of step (iv), and
- (vi) establishing antifungal activity of protein role by reverse genetics approach to obtain the novel protein from Bg_5672.
A process for preparing composition for antifungal activity, wherein the process comprising steps:
- (i) producing evolved Bg_9562 protein represented by sequence SEQ ID No. 2 in bulk quantities by fermentation in bioreactor;
- (ii) encapsulating the fermented product of step (i) in chitosan or non-chitosan based nanoparticles;
- (iii) using the encapsulated product of step (ii) to develop water base or oil base cream/ointment;
- and
- (iv) converting the product of step (iii) to antifungal peptide comprising films made from starch/oils.
Dependencies are inferred from the claim text.