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US 11,142,550 B2Granted
Split inteins with exceptional splicing activity
AssigneeThe Trustees of Princeton University
InventorsTom W. Muir, Adam J. Stevens, Neel H. Shah
Life of this patent
Estimating the term…
Abstract
Embodiments of the present invention relate to inteins, split inteins, compositions comprising inteins and methods for use of these.
Claims21
A split intein N-fragment comprising an amino acid sequence of at least 98% sequence identity to
- (SEQ ID NO:
- 1)
- CLSYDTEILTVEYGFLPIGKIVEERIECTVYTVDKNGFVYTQPIAQWHNR
- GEQEVFEYCLEDGSIIRATKDHKFMTTDGQMLPIDEIFERGL
- or to
- (SEQ ID NO:
- 2)
- CLSYDTEILTVEYGFLPIGKIVEERIECTVYTVDKNGFVYTQPIAQWHNR
- GEQEVFEYCLEDGSIIRATKDHKFMTTDGQMLPIDEIFERGLDLKQVDGL
- P.
7 more elements
A split intein C-fragment comprising an amino acid sequence of at least 98% sequence identity to
- (SEQ ID NO:
- 3)
- VKIISRKSLGTQNVYDIGVEKDHNFLLKNGLVASN,
- to
- (SEQ ID NO:
- 4)
- MVKIISRKSLGTQNVYDIGVEKDHNFLLKNGLVASN
- or to
- (SEQ ID NO:
- 389)
- VKIISRKSLGTQNVYDIGVGEPHNFLLKNGLVASN.
8 more elements
An intein comprises an amino acid sequence of at least 98% sequence identity to
- (SEQ ID NO:
- 390)
- CLSYDTEILTVEYGFLPIGKIVEERIECTVYTVDKNGFVYTQPIAQWHNRG
- EQEVFEYCLEDGSIIRATKDHKFMTTDGQMLPIDEIFERGLDLKQVDGLPV
- KIISRKSLGTQNVYDIGVEKDHNFLLKNGLVASN.
2 more elements
Dependencies are inferred from the claim text.