Cartridge and method of distributing biological sample in fluid channel thereof

A method of distributing a biological sample in a fluid channel of a cartridge, comprising:

• bonding a plate including a fluid inlet and a fluid outlet to a biochip to form a cartridge, wherein a fluid channel is formed between the biochip and the plate and has a boundary consisting of sidewalls of the plate and a top surface of the biochip, the fluid inlet and the fluid outlet are spaced apart in a first direction, the plate includes a top portion and a fence portion under and connected to the top portion, the top portion comprises only one protrusion protruding toward the biochip in a lengthwise direction of the top portion, a first sidewall of the protrusion meets a second sidewall of the fence portion to define a first acute angle therebetween and form a first gap, a third sidewall of the protrusion being opposite to the first sidewall of the protrusion meets a fourth sidewall of the fence portion to define a second acute angle therebetween and form a second gap, the first gap and the second gap are spaced apart in a second direction crossing the first direction, and the fluid channel includes a front portion, a middle portion and a rear portion arranged in sequence from the fluid inlet to the fluid outlet;
• injecting a biological sample through the fluid inlet to flow into the fluid channel in a direction, wherein a fluid flow of the biological sample is affected by the acute angle defined by the first and third sidewalls of the protrusion and the second and fourth sidewalls of the fence portion such that a portion of the biological sample near an edge of the fluid channel flows at a flow rate different from a flow rate of a portion of the biological sample near a center of the fluid channel;
• and
• injecting a liquid comprising a material immiscible with the biological sample through the fluid inlet to push the biological sample along the direction in the fluid channel.