Methods for manufacturing phthalocyanine dye conjugates and stable conjugates

A method of manufacturing a phthalocyanine dye-targeting molecule conjugate, the method comprising:

• (a) contacting a targeting molecule with a phthalocyanine dye under conditions to produce a conjugate comprising the phthalocyanine dye linked to the targeting molecule;
• (b) quenching by contacting with glycine;
• and
• (c) formulating the conjugate in a pharmaceutically acceptable buffer, wherein in each of steps (a)-(c):
• the only light to which the dye and conjugate are exposed has a wavelength within a range from at or about 400 nm to at or about 650 nm wherein the targeting molecule is an antibody or an antibody fragment.

A method of manufacturing a phthalocyanine dye-targeting molecule conjugate, the method comprising:

• (a) contacting a targeting molecule with a phthalocyanine dye under conditions to produce a conjugate comprising the phthalocyanine dye linked to the targeting molecule;
• (b) quenching by contacting with glycine;
• and
• (c) formulating the conjugate in a pharmaceutically acceptable buffer, wherein in each of steps (a)-(c):
• the only light to which the dye and conjugate are exposed has an intensity of less than at or about 200 lux wherein the targeting molecule is an antibody or an antibody fragment.