Universal donor checkpoint inhibitor silenced/gene edited cord blood killer cells
OwnerThomas Ichim, David Koos +1inventors
InventorsThomas Ichim, David Koos, Harry Lander
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Abstract
Disclosed are compositions of matters, cells, and treatment protocols useful for induction of anticancer responses in a patient suffering from cancer. In one embodiment the invention provides the use of NR2F6 silencing or gene editing in cord blood cells possessing anti-tumor activity in order to induce potentiated killer cells suitable for therapeutic use. In one embodiment said allogeneic cord blood killer cells are administered to initiate a cascade of antitumor immune responses, with initially responses mediated by allogeneic killer cells, and followed by endogenous immune responses.
Claims13
A method of inducing anticancer activity in mononuclear cells comprising the steps of:
- a) obtaining a cord blood sample;
- b) isolated mononuclear cells from said cord blood sample;
- c) culturing said mononuclear cells to induce gene silencing of the NR2F6 immunological checkpoint;
- and d) continuing said culture in the presence of IL-33 factor capable of endowing and/or augmenting said cord blood mononuclear cells with tumor cytotoxic activity.
Dependencies are inferred from the claim text.