Buoyancy-activated cell sorting (BACS)-compatible activation/transduction systems and methods
AssigneeThermoGenesis Corp.
InventorsPhilip H. Coelho, William Busa, Jonathan Ellis, Dalip Sethi
Life of this patent
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Abstract
Disclosed herein are methods for contacting in a closed container a host liquid including target cells, microbubble reagents comprising gas-core lipid-shelled microbubbles, and one or more antibodies or other ligands that bind to cell surface molecules on the target cells, wherein the one or more antibodies or other ligands are bound to the target cells or the microbubbles, wherein the contacting under conditions to produce target cells linked to microbubbles via the one or more antibodies or other ligands and activating the target cells to generate activated target cells.
Claims24
A method for isolation and activation of target cells, comprising
- (a) contacting in a closed container a host liquid comprising the target cells with gas-core lipid-shelled microbubbles and one or more antibodies that bind to cell surface molecules on the target cells, wherein the one or more antibodies are bound to the target cells or the microbubbles, and wherein the contacting produces target cells linked to the microbubbles via the one or more antibodies;
- (b) isolating in the closed container the target cells linked to microbubbles from the other cells in the host liquid to produce isolated target cells linked to microbubbles;
- (c) collapsing and/or disrupting in the closed container the microbubble component of the isolated target cells linked to microbubbles between about 1 minute and about 180 minutes after initiating the contacting in step (a), wherein the collapsing and/or disrupting produces isolated target cells linked to microbubble residua;
- and
- (d) culturing the isolated target cells linked to microbubble residua to produce activated target cells via the one or more antibodies linked to the microbubble residua.
Dependencies are inferred from the claim text.