Method for discriminating microorganism
AssigneesShimadzu Corporation, Meijo University
InventorsHiroto Tamura, Naomi Yamamoto, Teruyo Kato, Keisuke Shima, Shinji Funatsu
Life of this patent
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Abstract
To provide a method for discriminating a microorganism including: a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum; a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum; and a discrimination step of discriminating which bacterial species of Escherichia coli, Shigella bacteria, and Escherichia albertii the microorganism contained in the sample contains based on the mass-to-charge ratio m/z, in which at least one of 13 ribosomal proteins S5, L15, S13, L31, L22, L19, L20, L13, S15, L25, HNS, HdeB, and L29 is used as the marker protein.
Claims4
A method for discriminating a microorganism, the method comprising:
- a) a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum;
- b) a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum;
- and
- c) a discrimination step of discriminating which bacterial species of Escherichia coli, Shigella bacteria, and Escherichia albertii is contained in the sample based on the mass-to-charge ratio m/z,
- wherein
- at least one of 13 ribosomal proteins S5, L15, S13, L31, L22, L19, L20, L13, S15, L25, HNS, HdeB, and L29 is used as the marker protein, and
- the Shigella bacteria is discriminated based on the mass-to-charge ratio m/z of at least one of ribosomal proteins L29 and L13 and ribosomal proteins L31 and L13.
A method for discriminating a microorganism, the method comprising:
- a) a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum;
- b) a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum;
- and
- c) a discrimination step of discriminating which bacterial species of Escherichia coli, Shigella bacteria, and Escherichia albertii is contained in the sample based on the mass-to-charge ratio m/z,
- wherein
- at least one of 13 ribosomal proteins S5, L15, S13, L31, L22, L19, L20, L13, S15, L25, HNS, HdeB, and L29 is used as the marker protein, and
- Shigella flexneri of the Shigella bacteria is discriminated based on the mass-to-charge ratio m/z of at least one of ribosomal proteins L31 and L29.
A method for discriminating a microorganism, the method comprising:
- a) a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum;
- b) a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum;
- and
- c) a discrimination step of discriminating which bacterial species of Escherichia coli, Shigella bacteria, and Escherichia albertii is contained in the sample based on the mass-to-charge ratio m/z,
- wherein
- at least one of 13 ribosomal proteins S5, L15, S13, L31, L22, L19, L20, L13, S15, L25, HNS, HdeB, and L29 is used as the marker protein, and
- Shigella sonnei of the Shigella bacteria is discriminated based on the mass-to-charge ratio m/z of at least a ribosomal protein L13.
A method for discriminating a microorganism, the method comprising:
- a) a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum;
- b) a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum;
- and
- c) a discrimination step of discriminating which bacterial species of Escherichia coli, Shigella bacteria, and Escherichia albertii is contained in the sample based on the mass-to-charge ratio m/z,
- wherein
- at least one of 13 ribosomal proteins S5, L15, S13, L31, L22, L19, L20, L13, S15, L25, HNS, HdeB, and L29 is used as the marker protein, and
- Shigella boydii of the Shigella bacteria is discriminated based on the mass-to-charge ratio m/z of at least one of ribosomal proteins L25, HNS, and L13.
Dependencies are inferred from the claim text.