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US 11,136,608 B2Granted
Microbial fermentation method for production of n-acetyl-d-glucosamine and/or d-glucosamine salt
OwnerLan Suninventor
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Abstract
This Invention discloses a method for production of N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt by microbial fermentation. The method is intended to manufacture N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt in higher efficiency and higher yield, by expression of vitreoscilla hemoglobin in microorganism.
Claims6
A method for production of N-Acetyl-D-Glucosamine and/or D-Glucosamine by microbial fermentation, including:
- a) Cultivation of a microorganism in the fermentation medium, where the said microorganism is transformed with a modified recombinant nucleic acid molecule encoding modified vitreoscilla hemoglobin (Vhb);
- wherein the modified nucleotide sequence encoding the said modified vitreoscilla hemoglobin (Vhb) contains a genetic modification to increases the activities of the vitreoscilla hemoglobin (Vhb) of the amino acid sequence of SEQ ID NO:
- 61;
- where the said modified vitreoscilla hemoglobin (Vhb) contain substitutions at the following sites of the amino acid sequence SEQ ID No:
- 61:
- methionine at site 45 is substituted by leucine, cysteine at site 86 is substituted by glycine, and tyrosine at site 95 is substituted by serine;
- and
- b) Collection of N-Acetyl-D-Glucosamine produced from the cultivation step a).
5 more elements
Dependencies are inferred from the claim text.