Method for production of reprogrammed cell using chromosomally unintegrated virus vector
AssigneeID Pharma Co., Ltd.
InventorsNoemi Fusaki, Hiroshi Ban, Mamoru Hasegawa, Yoshikazu Yonemitsu
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Abstract
An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.
Claims6
A Sendai virus vector having a deletion of F gene, and encoding an M protein having mutations of G69E, T116A, and A183S, an HN protein having mutations of A262T, G264R, and K461G, a P protein having mutations of L511F, and a L protein having mutations of N1197S and K1795E, wherein viral protein(s) encoded in said Sendai virus vector further comprises mutations of any one of (i) to (iv) below:
- (i) D433A, R434A and K437A in the P protein, and L1361C and L1558I in the L protein
- (ii) D433A, R434A and K437A in the P protein;
- (iii) Y942H, L1361C and L1558I in the L protein;
- and
- (iv) D433A, R434A and K437A in the P protein, and L1558I in the L protein,
- and wherein the Sendai virus vector can be used for expressing reprograming factor genes in a somatic cell to induce an induced pluripotent stem cell.
Dependencies are inferred from the claim text.