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US 11,136,588 B2Granted
Method for producing whole plants from protoplasts
AssigneesInstitute for Basic Science, Seoul National University R&Db Foundation, Aict
InventorsJin Soo Kim, Jungeun Kim, Sunghwa Choe, Je Wook Woo, Soon Il Kwon
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Abstract
The present invention relates to method for preparing a plant from a protoplast comprising knocking-out or knocking-in one or more the endogenous gene of the protoplast, and the plant regenerated from a genome-modified protoplast prepared by the above method.
Claims9
A method for preparing a plant from a protoplast comprising knocking-out an endogenous gene of the protoplast by introducing a pre-assembled Cas protein-guide RNA ribonucleoprotein(RNP),
- wherein the guide RNA specifically binds to a PHYB (phytochrome B) gene or a BIN2 (bridging integrator 2) gene, and
- wherein the guide RNA specifically binding to PHYB (phytochrome B) is encoded by a nucleic acid comprising the sequence of SEQ ID No:
- 85;
- or the guide RNA specifically binding to BIN2 (bridging integrator 2) is encoded by a nucleic acid comprising the sequence of SEQ ID No:91.
1 more element
Dependencies are inferred from the claim text.