Engineered phenylalanine ammonia lyase polypeptides
AssigneeCodexis, Inc.
InventorsGjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
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Abstract
The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
Claims33
An engineered polypeptide comprising an amino acid sequence having at least 90% sequence identity to reference sequence SEQ ID NO:10, wherein said amino acid sequence comprises an amino acid residue selected from leucine, methionine, and glutamine at position 305, and wherein said engineered polypeptide further comprises at least one or more of the following substitution sets selected from 32/54/73/305/503/521/565, 54/59/73/305/503/521/565, and 54/59/305/503/521/565, wherein said positions in said amino acid sequence are in reference to SEQ ID NO:10.
Dependencies are inferred from the claim text.